My Life With Science!

Despite this exemplary academic trajectory, what makes Dorly a special human being are her unique qualities: loyalty, courage, tenderness, and tenacity. Woman, mother, sister and friend, she is endowed with profound sensitivity, a conciliatory spirit and the ability to aggregate people and ideals around a greater good. Her respect for life, in all its forms, has always guided her in search of health promotion and a better life for all. And so, in its continuous overcoming of challenges, guided by an acute scientific curiosity and its non-conformity with the paradigms and limitations of traditional medicine, in an untiring spirit and an unwavering purpose of serving collective interests, firmly carried out research based on highly diluted drugs and complex biological response modifiers. A significant part of the results of his efforts and his holistic view is presented in this book which, as an author, proposes to contribute to life, health and the construction of happiness for all.

The presence of the unexpected in my life!

Dra. Dorly de Freitas Buchi, the renowned Brazilian researcher, holds a Ph.D. in Biological Sciences (Biophysics) from the Federal University of Rio de Janeiro. His solid academic background is reflected in the publication of several scientific articles in the field of Cell Biology. Professor, for more than 40 years at the Federal University of Paraná, she combines extensive experience in higher education, both in undergraduate and graduate courses, and effective contributions in the sphere of the university administration. Its orientation activities are remarkable, from scientific initiation to doctorate, having promoted the training of a large number of young scientists.

Francine Bittencourt Potrich, Carolina Camargo de Oliveira, Edvaldo Da Silva Trindade  –Federal University of Paraná

Our research team at Federal University have studied in vitro and in vivo cancer cells treated with homeopathy in the last 20 years. After using different products and analyzing the different results carefully, we arrived at two complexes produced with homeopathic techniques, coded as M1 and M8. The results obtained using these two products were surprising and some were even published in scientific journals with good impact.

Melanoma is a highly metastatic skin cancer type. Conventional chemotherapy drugs currently in used are of limited value, thus more effective therapies are needed. This work aimed at investigation and elucidation of possible changes in tumor cell features triggered by highly diluted natural complex (M1 and M8) in vitro treatment using melanoma cells.

For this several parameters were analyzed, focusing on possible changes in mRNA expression of invasion related proteins, migration and proliferation; and evaluation of reactive species; melanogenesis and multidrug resistance mechanism. Finally, glycoconjugates evaluation was performed.

The results clearly demonstrated that M1 and M8 treatment accounts to reduction in metastasis related features important by decreasing metaloproteases gene expression (MT1-MMP, MMP-2 and MMP-9), and your gelatinase activity, as well as cell migration. The latter was, possibly due to increased reactive oxygen (ROS), release of nitric oxide (NO) and reduction intracellular NO. Interestingly this effect was accompanied by melanin synthesis reduction and activity and expression reduction of tyrosinase (TYR), as well as TYRP-1, TYRP-2 and MITF expression. Finally, was observed reduction of drugs resistance mechanism (ABC transporters). As for glycoconjugates, there was a clear reduction in the secretion of hyaluronic acid and the gene expression of hyaluronidases. Still, it was evident the reduction of gene expression of proteoglycans and heparan sulfate enzymes modifiers. Taken together these findings suggest that metastatic phenotypic characteristics are modified by M1 and M8 treatment, justifying previous in vitro and in vivo findings.

Thus, these complexes can be considered as promising adjuvant cancer therapy candidates.

Francine Bittencourt Potrich, 2014, PhD thesis