My Life With Science!

Despite this exemplary academic trajectory, what makes Dorly a special human being are her unique qualities: loyalty, courage, tenderness, and tenacity. Woman, mother, sister and friend, she is endowed with profound sensitivity, a conciliatory spirit and the ability to aggregate people and ideals around a greater good. Her respect for life, in all its forms, has always guided her in search of health promotion and a better life for all. And so, in its continuous overcoming of challenges, guided by an acute scientific curiosity and its non-conformity with the paradigms and limitations of traditional medicine, in an untiring spirit and an unwavering purpose of serving collective interests, firmly carried out research based on highly diluted drugs and complex biological response modifiers. A significant part of the results of his efforts and his holistic view is presented in this book which, as an author, proposes to contribute to life, health and the construction of happiness for all.

The presence of the unexpected in my life!

Dra. Dorly de Freitas Buchi, the renowned Brazilian researcher, holds a Ph.D. in Biological Sciences (Biophysics) from the Federal University of Rio de Janeiro. His solid academic background is reflected in the publication of several scientific articles in the field of Cell Biology. Professor, for more than 40 years at the Federal University of Paraná, she combines extensive experience in higher education, both in undergraduate and graduate courses, and effective contributions in the sphere of the university administration. Its orientation activities are remarkable, from scientific initiation to doctorate, having promoted the training of a large number of young scientists.


Metastatic melanoma is the most aggressive form of skin cancer, and the most rapidly expanding cancer in terms of worldwide incidence, with a 5 years survival rate of less than 10% after metastasis establishment. Chemo and radiotherapeutic approaches in melanoma treatment have been shown disappointing results, also resulting in severe adverse effects. In this work we have evaluated M1 treatment, a highly diluted natural complex: 1) in vitro, in a co-culture model between human mononuclear cells obtained from leukoreduction chambers (LRS chambers) after plateletpheresis procedure, and a human metastatic cell lineage (1205Lu), and 2) in vivo, in C57BL/6 mice bearing melanoma tumors (B16-F10 cells subcutaneously inoculated into dorsal flanks).

Treatment was added to mononuclear cells culture on in vitro assays and giving by inhalation for 10 min/day during 14 days on in vivo assays. We have obtained the following results 1) in vitro treatment have shown that mononuclear cells previously treated for 48 hours with M1 and then submitted to co-culture, reduced proliferation rate, induced cell cycle arrest, increased apoptosis and decreased nitrogen and oxygen reactive species release from 1205Lu tumor cells.

A decrease in cell invasion and lower beta-catenin expression was also observed. Besides that, when mononuclear cells were analyzed by flow cytometry, an increase in CD3-/CD56+ Natural Killer cell population was seen in the treated group. Finally, when isolated from mononuclear cultures, and co-cultured with 1205Lu, those Natural Killer cells from M1 treated group, showed a significant increase in cytotoxicity against melanoma cells. 2) in vivo treatment: after 14 days of mice M1 treatment by inhalation, tumors were removed, and histopathology analysis was made using color deconvolution plugin and area measurement from ImageJ software.

Both tumor size and weight were reduced on treated animals. Histopathology investigation showed a higher tumor cell death whereas lower microvascular density, which may be related to the lower amount of host angiotensin II type 1 receptor positive cells on tumor periphery was found on treated animals. Since tumor growth depends on nutrients carried out by blood, inhibition of angiogenesis has been emerging as a new therapeutic goal for treating cancer.

Previous results showing decreasing in NF-κB in human carcinoma colon-rectal cells (BMC CAM 2011, 11:101) together with these data allowed us to conclude that the natural complex M1 may have an important contribution in reducing noninvasive tumor burden. Also, it was an effective and nontoxic approach to improve anti-tumor effects. Finally, not only decreasing neovascularization, increasing NK cells but increasing activation of the NK cells, it could be used as adjuvant therapy in tumors.

Diogo Kuczera, Lucas Ferrari de Andrade, Carolina Camargo de Oliveira, Edvaldo da Silva Trindade and Dorly de Freitas Buchi

 Departamento de Biologia Celular, SCB, Universidade Federal do Paraná, Brazil

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