My Life With Science!

Despite this exemplary academic trajectory, what makes Dorly a special human being are her unique qualities: loyalty, courage, tenderness, and tenacity. Woman, mother, sister and friend, she is endowed with profound sensitivity, a conciliatory spirit and the ability to aggregate people and ideals around a greater good. Her respect for life, in all its forms, has always guided her in search of health promotion and a better life for all. And so, in its continuous overcoming of challenges, guided by an acute scientific curiosity and its non-conformity with the paradigms and limitations of traditional medicine, in an untiring spirit and an unwavering purpose of serving collective interests, firmly carried out research based on highly diluted drugs and complex biological response modifiers. A significant part of the results of his efforts and his holistic view is presented in this book which, as an author, proposes to contribute to life, health and the construction of happiness for all.

The presence of the unexpected in my life!

Dra. Dorly de Freitas Buchi, the renowned Brazilian researcher, holds a Ph.D. in Biological Sciences (Biophysics) from the Federal University of Rio de Janeiro. His solid academic background is reflected in the publication of several scientific articles in the field of Cell Biology. Professor, for more than 40 years at the Federal University of Paraná, she combines extensive experience in higher education, both in undergraduate and graduate courses, and effective contributions in the sphere of the university administration. Its orientation activities are remarkable, from scientific initiation to doctorate, having promoted the training of a large number of young scientists.

Background: Leishmaniasis is a major vector-borne metazoonosis disease, caused by obligate intramacrophage protozoa of the genus Leishmania. Their exclusively intracellular life style suggests that the amastigotes possess mechanisms to avoid killing by host cells. Cutaneous leishmaniasis, the most common form of the disease, causes ulcers on exposed parts of the body, leading to disfigurement, permanent scars, and stigma and in some cases disability. Many studies concluded that the cytokines profile of host immune system has fundamental role in humans and animals natural self-healing. In this study we detected and quantified the cytokines profile produced by macrophages during Leishmania amazonensis interaction after Thuya or M1 treatment.

Methodology: Cells of murine strain RAW 264.7 (ATCC:TIB -71) were treated with Thuya 30 cH or with the M1complex during 48 hours, and allowed to interact with cultured Leishmania amazonensis strain WHOM/BR/75/Josefa for 2 and for 18 hours. The supernatant aliquots were processed in a Luminex MAGPIX® (EMD Millipore®) apparatus, using a MILLIPLEX MAG PIX for 13 cytokines kit, according to the manufacturers’ instructions. IL1α, IL1β, TNFα, IL 6, IL 10, IL12 p40, IL 12 p70, MIP-1β, INFγ, MCP-1, RANTES/CCL 5, GM-CSF and VEGF-α were measured, in duplicate samples. Results were expressed in pg/mL. Samples with quantification below the detection limit for the analysis were registered as “zero” and samples above the quantification limit of the standard curb were registered with the value equal to the highest value of the curb.

Results: the treatment with Thuya, after 18 hours modified 5 from 13 cytokines tested: increased IL 12 p70, and IL1β; decreased MCP-1, TNFα, and INFγ. The treatment with M1 complex altered 6 from 13 cytokines tested: increased IL1α, IL 10, IL 12 p70, INFγ, and MCP-1; and decreased MIP-1β. The main results were resumed in Figure 1.


Fig.1: Main results signaling cytokines whose quantification has changed after treatment with Thuya and M1, indicating also the main functions of each one.