My Life With Science!

Despite this exemplary academic trajectory, what makes Dorly a special human being are her unique qualities: loyalty, courage, tenderness, and tenacity. Woman, mother, sister and friend, she is endowed with profound sensitivity, a conciliatory spirit and the ability to aggregate people and ideals around a greater good. Her respect for life, in all its forms, has always guided her in search of health promotion and a better life for all. And so, in its continuous overcoming of challenges, guided by an acute scientific curiosity and its non-conformity with the paradigms and limitations of traditional medicine, in an untiring spirit and an unwavering purpose of serving collective interests, firmly carried out research based on highly diluted drugs and complex biological response modifiers. A significant part of the results of his efforts and his holistic view is presented in this book which, as an author, proposes to contribute to life, health and the construction of happiness for all.

The presence of the unexpected in my life!

Dra. Dorly de Freitas Buchi, the renowned Brazilian researcher, holds a Ph.D. in Biological Sciences (Biophysics) from the Federal University of Rio de Janeiro. His solid academic background is reflected in the publication of several scientific articles in the field of Cell Biology. Professor, for more than 40 years at the Federal University of Paraná, she combines extensive experience in higher education, both in undergraduate and graduate courses, and effective contributions in the sphere of the university administration. Its orientation activities are remarkable, from scientific initiation to doctorate, having promoted the training of a large number of young scientists.

Cytokine is a general term for a large group of molecules involved in signaling between cells during immune responses and are produced by different cell types. Its production triggers a cascade of reactions, influences the synthesis of other cytokines and can exert regulatory mechanisms for the immune and inflammatory responses.

Bacteria or bacterial cell wall products (such as LPS) stimulate monocytes/macrophages to produce both pro-inflammatory and TNF-a cytokines, as well as anti-inflammatory cytokines such as IL-10. Modulation of the production of cytokines by active substances highly diluted and dynamized has shown increased interest due to its efficiency and low toxicity. The objective of this study was to evaluate the production of TNF-a and IL-10 by human monocytes stimulated with LPS and treated with homeopathic complexes (HM-LPS) in vitro.

The homeopathic complexes designated Chelidonium majus and associations (M1) and Calcarea carbonica and associations (M8) were tested on human monocytes obtained from three healthy donors, which were purified by adhesion and cultured in 96-well plates (1×105 cells/ml) in RPMI-1640 medium, supplemented with antibiotics and 5% autologous human serum, and stimulated with 5mg / ml LPS. The HM-LPS were treated with 20% M1 or M8 and maintained at 37 ° C and 5% CO2 for 24h. The concentration of TNF-a and IL-10 in the cell culture supernatant was assessed by flow cytometry using BD-Cytometric Bead Array (BD-CBA) technology.

Figure 1 and 2 – Treated monocytes observed at Scanning Electron Microscope in different magnifications.

The treatment of HM-LPS with M1 statistically significantly reduced the production of TNF-a, whereas with M8 what was observed was a tendency to decrease in the production of this cytokine. The concentration of IL-10 did not show the difference after treatment with both drugs. These results suggest that M1, more than M8, can modulate the production of TNF-a and IL-10 by HM-LPS, indicating a potential anti-inflammatory action.

Da Lozzo, E.J.; Gonçalves, J.P.; Kuczera, D.; Buchi, D. F.

Universidade Federal do Paraná, Departamento de Biologia Celular, SCB

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